Most, but not all, yeast strains in the deletion library contain the [PIN(+)] prion
Yeast · 2010
Early research: almost all strains of a widely used yeast gene-deletion library carry the [PIN+] prion, which can affect large genetic screens.
Research question
Do strains in the yeast deletion library carry the [PIN+] prion, and which genes are required to maintain it?
Clinical & scientific significance
Alerts researchers that a non-Mendelian element is present across a resource used in many genome-wide studies.
Key findings reported in the paper
- Over 97% of deletion strains were [PIN+].
- Only deletions of HSP104 or RNQ1 prevented [PIN+] maintenance, indicating 5,153 non-essential genes are not required for propagation.
Objectives
To survey [PIN+] status across the deletion library.
Methods
Screening of deletion strains using an RNQ1-GFP fluorescent reporter and maintenance tests of three [PIN+] variants.
This summary is based only on the published abstract.
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Authors
Manogaran AL, Fajardo VM, Reid RJ, Rothstein R, Liebman SW
Citation
Most, but not all, yeast strains in the deletion library contain the [PIN(+)] prion. Yeast. 2010; 27(3):159-66. doi:10.1002/yea.1740. PMID: 20014044.
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